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- W2352025507 abstract "Objective To find an effective technique for isolation,cultivation and purification of Schwann cells(Schwann cells,SCs)from adult rabbit peripheral nerves,discuss the biological effects of basic fibroblast growth factor(b-FGF)and nerve growth factor(NGF)to SC cultured in vitro.Methods Adult predegenerated rabbit sciatic nerves were cultured in DMEM medium by modified repeated explanation of adult SCs.The purity of SCs was identified through the phase contrast microscope and the growth and the biological characteristics of SC were observed by cell morphological survey,cell growth curve and S-100 stains.Results In these methods which could inhibit fibroblasts.b-FGF was able to promote the proliferation of SC;S-100 stain showed that the purity of SC population was up to 94%.Conclusion 1.Predegenerated nerve,b-FGF and NGF could promote the proliferation of SC.2.Plenty and purified SCs could be obtained with such methods.Schwann Cells harvested from rabbit can be used as seeded cells in peripheral nerve tissue engineering." @default.
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- W2352025507 date "2008-01-01" @default.
- W2352025507 modified "2023-09-23" @default.
- W2352025507 title "The Experimental Study of Purifying Cultured Schwann Cells from Adult Rabbit in Vitro" @default.
- W2352025507 hasPublicationYear "2008" @default.
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