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- W2352034114 abstract "Aim To investigate the biological effects of ectopically expressing a constitutively active transcription factor Gli2(aGli2) in murine astrocytes.Methods A lentivirus vector which could express aGli2 was constructed.Lentivirus was packaged,the murine astrocytes were infected,and biological effects were indentified.Results The astrocytes with ectopic aGli2 obtained the ability to form neurospheres after 13 days’ culture in neural stem cell medium.Structurally,the neurospheres derived from aGli2 astrocytes were indistinguishable from normal murine neurosphere.Immunofluorescence staining demonstrated that these cells expressed Nestin,the neural stem cell marker.Conclusion Ectopic expression of constitutive active Gli2 could make the murine astrocytes obtain the ability to form neurospheres." @default.
- W2352034114 created "2016-06-24" @default.
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- W2352034114 date "2013-01-01" @default.
- W2352034114 modified "2023-09-28" @default.
- W2352034114 title "The biological effects of ectopically expressing constitutively active type Gli2 on murine astrocytes" @default.
- W2352034114 hasPublicationYear "2013" @default.
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