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- W2352040644 abstract "Objective To establish an improved method for isolation and purification of sialoprotein associated with cones and rods(SPACR)in interphotoreceptor matrix(IPM).Methods Protein in PBS-insoluble IPM was extracted,SPACR was purified by DEAE-Sephacel and Sephacryl S-300 HR chromatography.Results The map of 2D gel electrophoresis showed that SPACR was in the section of pI 5-5.5 which the relative molecular weight was 150kDa.Purified SPACR was obtained.Conclusion A purification procedure was established in this study for SPACR with highly efficiency and simplicity by DEAE-Sephacel and Sephacryl S-300 HR chromatography.The present results would be valuable in analyzing biological activities and structural determination of SPACR." @default.
- W2352040644 created "2016-06-24" @default.
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- W2352040644 date "2011-01-01" @default.
- W2352040644 modified "2023-09-24" @default.
- W2352040644 title "Isolation and Purification of chick SPACR" @default.
- W2352040644 hasPublicationYear "2011" @default.
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