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- W2352042007 abstract "The α-amylase structural gene of Bacillus Licheniformis mAn-612 was successfully cloned by PCR method. The experiments showed that the α-amylase structure gene is 1449bp.The ligation mixture was transformed into DH5α( the cloning host) after it was ligated with pUCm-T vectors for more than one hour. Positive cloning was obtained when we cleaved with restriction enzyme NcoI and XhoI .Plasmids DNAs was cleaved with NcoI and XhoI ,and then ligated with pET-28a(+) cleaved with NcoI and XhoI .The ligated DNA was used to transformed into DE3. The positive recombinant transformant was incubated with IPTG .The foreign gene is expressed efficiently in DE3." @default.
- W2352042007 created "2016-06-24" @default.
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- W2352042007 date "2004-01-01" @default.
- W2352042007 modified "2023-09-25" @default.
- W2352042007 title "Cloning and Expression of α-amylase Gene from Bacillus Licheniformis mAn-612" @default.
- W2352042007 hasPublicationYear "2004" @default.
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