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- W2352121257 abstract "In order to establish a stability and repeatability reaction system of wild Camellia meiocarpa, fi ve factors, the conc entration of DNA template, primers, dNTPs, Mg2+ and Taq DNA polymerase, were studied by the single factor tests, orthogonal experimental design L16(45)and variance analysis, with the total DNA of Camellia meiocarpa as an experimental material. The PCR amplifi cation reaction proces s was optimized. The optimal system of ISSR-PCR reaction system was established as follows: 30 ng DNA temple, 0.7 μmo l/L primers, 0.25 mmol/L dNTPs, 2.0 mmol/L Mg2+, 1.75U Taq DNA polymerase were contained in 20 μL reaction system. The variance analysis shows that the effects of 5 factors on the system did not reach a signifi cant level, and of them, the primer concentration had the greatest impact on the reaction system. The established system was appli ed in the amplifi cation s of 5 varieties of wild Camellia meiocarpa Hu, and the results indicated the system had stability and repeatability." @default.
- W2352121257 created "2016-06-24" @default.
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- W2352121257 date "2014-01-01" @default.
- W2352121257 modified "2023-09-26" @default.
- W2352121257 title "Establishment and optimization of ISSR-PCR reaction system of Camellia meiocarpa Hu." @default.
- W2352121257 hasPublicationYear "2014" @default.
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