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- W2352132023 abstract "Objective To construct lentiviral vector carrying STAT3 and mutated mouse oestrogen receptor α(ER) transinfected into neurons of rat dorsal root ganglia(DRG) to investigate functions of STAT3 in adult rat primary sensory neurons.Methods Gene recombinant technology was employed to cleave and insert the PmeI fragment of STAT3ERIRES-GFP into the PmeI site of the lentivirus transfer vector pRRL-MCS+ to construct a lentiviral vector pRRLSTAT3ER-IRES-GFP.RT-PCR,Western blotting and sequencing analysis were used for identification.Then,293T cells were transfected with main vector pRRL-STAT3ER-IRES-GFP,packaging plasmid pCMVdeltaR8.74 and coated plasmid VSV-G.Lentiviral vectors were packaged and the titer was determined.The peripheral and dorsal roots of rat left L5 DRG were cut near their ganglia,L5 DRGs were taken to separate neurons cultured in vitro.The constructed pRRL-STAT3ERIRES-GFP was used to transfect neurons and nuclear translocation of STAT3ER and neurite lengths of neurons were analyzed.Results The lentiviral vector plasmid pRRL-STAT3ER-IRES-GFP was identified correctly by RT-PCR and Western blotting,and DNA sequencing analysis confirmed that STAT3 gene sequencing was exactly the same with that reported by Genbank.The concentration of the virus titer was 10 10-11 TU /mL after transinfection of 293T cells.After transinfection with pRRL-STAT3ER-IRES-GFP,nuclear translocation of STAT3ER in transfected neurons was seen obviously after stimulation with 4HT,neurite outgrowth was enhanced by STAT3.Conclusion Lentiviral vector carrying STATSER was successfully constructed and activation of STAT3 signaling is beneficial to axonal growth." @default.
- W2352132023 created "2016-06-24" @default.
- W2352132023 creator A5003870203 @default.
- W2352132023 date "2010-01-01" @default.
- W2352132023 modified "2023-09-25" @default.
- W2352132023 title "Construction of lentivirus vectors carrying STAT3ER and its effect on neurite growth" @default.
- W2352132023 hasPublicationYear "2010" @default.
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