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- W2352154288 abstract "Objective To identify potential influence of glycerin on mouse acute toxicity test of Dermatophagoides Der.pterronyssinus skin prick test(SPT) solution.Methods Stock extract of Der.pterronyssinus SPT solution was prepared in the ratio of 1:10(W/V) with extract solution without phenol.Stock extract was sterilized with 0.4μm filters.Thirty five BALB/c mice were divided into 5 groups with abdominal cavity injection of 0.5 ml.Group A:stock extract with 0.4%phenol(3 ml) +glycerin(3 ml);group B:stock extract(3 ml) +0.9%sodium chloride injection(NS)(3 ml);group C:glycerin(3 ml) +NS(3 ml); group D:NS with 0.4%phenol(6 ml);group E:NS(6 ml).Autopsy was done for each group.Results All the mice in Group A and C shown decreased activities and their tails cocked up in about 10 minutes after injection.Then,they dithered,and rolled about.All mice in group A and C died within 25 minutes.Autopsy was done immediately,gut edema and ascites could be found.Pulmonary congestion was shown complicated with thickening of alveolar wall,congestion of capillary and lymphocytes infiltration in pulmonary interstitial tissue.Intestinal wall shown edema,mucosa epithelia degeneration,necrosis and excoriation.No abnormal change was shown in liver,spleen and kidney.All mice in Group B,D and E shown normal activities and the biopsy was done after one week.No abnormal phenomena was seen in lung,liver,spleen and kidney.Conclussion Stock extract of Der.pterronyssinus without glycerin should be used in mice acute toxicity test of Der.pterronyssinus SPT solution." @default.
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- W2352154288 date "2008-01-01" @default.
- W2352154288 modified "2023-09-25" @default.
- W2352154288 title "Influence Glycerin on Mice Acute Toxicity Test of Dermatophagoides Pterronyssinus Skin Prick Test Solution" @default.
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