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- W2352176312 abstract "Objective To construct the recombinant vectors that coexpress wild type p53(wt-p53) and siRNA-mdm2 for gene therapy in androgen independent prostate cancer.Methods The subcloning,T-Acloning,and PCR technique were used to construct the recombinant vectors,named pcDNA3.1-p53,siRNA-mdm2,p53/siRNA-mdm2,and siRNA-scramble.The recombinant expression vectors mentioned above were transfected into PC-3 cells,the expression levels of the mdm2 siRNA and p53 after transfection were detected by the semi-quantitative RT-PCR analysis and Western blotting analysis.MTT assay was used to detect the proliferation inhibition of PC-3 cells after transfection.Results The recombinant plasmids containing both wt-p53 and siRNA-mdm2 were successfully constructed,and confirmed by DNA sequence analysis.After transfected for 48 h,the expression of GFP was observed.The expression level of wt-p53 was increased,and the expression of mdm2 was decreased detected by RT-PCR and Western blotting.MTT assay showed that the inhibitory rate of proliferation of PC-3 cells was 40.4% after transfection.Conclusion The recombinant plasmids containing both wt-p53 and siRNA-mdm2 are successfully constructed,they can remarkablely inhibit the proliferation of PC-3 cells." @default.
- W2352176312 created "2016-06-24" @default.
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- W2352176312 date "2010-01-01" @default.
- W2352176312 modified "2023-09-26" @default.
- W2352176312 title "Construction of vectors encoding both wide type p53 protein and siRNA-mdm2 and their expressions in PC-3 cells" @default.
- W2352176312 hasPublicationYear "2010" @default.
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