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- W2352188270 abstract "Objective Molecular cloning and sequencing of the antianiogenic peptide alphastatin gene.Methods According to sequence of mature alphastatin supplied by GenBank,a double strand cDNA of mature alphastatin was chemically synthesized by using asymmetrical primer/template method which included a restrict enzyme side on the two extremmation and cloned into the pGEM-T Easy.pGEM-T/Al was confirmed by endonuclease analysis and sequencing.Results Alphastatin gene was successfully synthesized chemically in vitro and cloned into plasmid pGEM-T Easy.The sequencing result of positive clone was identical to what sequenced and supplied by GenBank.Conclusion By cloning the alphastatin gene,the sequencing result of positive clone is identical to what sequenced and supplied by GenBank.It has paved the way for further study on gene therapy of tumor." @default.
- W2352188270 created "2016-06-24" @default.
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- W2352188270 date "2007-01-01" @default.
- W2352188270 modified "2023-09-27" @default.
- W2352188270 title "Cloning and sequencing of antianiogenic peptide alphastatin gene" @default.
- W2352188270 hasPublicationYear "2007" @default.
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