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- W2352191950 abstract "Objective In order to identify the changes of cellular immune functions of BALB/c mice after immunization with different HBsAg antigens. Methods By using plasma , CHO and yeast derived HBsAg as antigenes, splenic mononuclear cells (MNC) were respectively prepared at 5 , 10 , 15 , 20 and 25 days after immunization. The proliferation of MNC induced by ConA or LPS was assayed. The delayed type hypersensitivity (DTH), as determined by footpad swelling using sheep red blood cells (SRBC) as sensitinogen, was also studied in these immunized mice. Results After immunization, each kind of HBsAg showed a specific time activity curve. Generally, the stimulation index (SI) obviously increased at 20 th or 25 th day. Higher SI value was observed in plasma HBsAg immunized animals than in yeast HBsAg group. Hansenula HBsAg significantly enhanced the proliferation of MNC even at 5 th day. Immunization with CHO HBsAg obviously inhibited DTH reactivity ( P 0.05 ). Conclusion HBsAg antigens from different sources induce different types of immunological reactivities in varying degrees. CHO HBsAg inhibit T H1 cell mediated DTH reactivity. [" @default.
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- W2352191950 date "2001-01-01" @default.
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- W2352191950 title "The effects of immunization with hepatitis B surface antigen on cellular immunity of BALB/c mice" @default.
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