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- W2352198342 abstract "Grass carp (Ctenopharyngoden idellus) is an economically important fresh water fish in China. Although a significant amount of effort has been devoted to study the development of vaccines against infectious diseases in this fish, little was known about the biological features of its immunoglobulin. We induced the immune response in Grass carp with the vaccine consisting of inactivated bacterial Aeromonas hydrohila. The increased level of immunoglobulin in response to the vaccination challenge was observed. The immunological and biochemical features of the immunoglobulin protein was characterized. We used one year fish C. idellus of 100 to 150 grams of body weight. Each fish in the experimental group received A. hydrophila vaccine (strain TPS 30 one billion inactivated bacteria per ml) at a dose of 0.5 ml. Vaccine solution was injected into the fish muscle under the back fins. Controlled group of fish received no treatment, but were raised for the same period of time. Sever and 14 days after the first injection, blood was collected from the tail arteries and sera was separated. The activity and specificity of immunoglobulin in fish sera were tested with standard agglutination assays conventionally. Immunoglobulin proteins was isolated from fish sera either by the fractionation of saturated ammonium sulfate or by Sephadex G 200 gel column chromatography and by high pH discontinuous polyacrylamide gel electrophoresis. Antigen specific agglutination testing revealed that the highest antibody titer was present in the fraction precipitated with 35% saturated ammonium sulfate, or in the first elution peak of chromatography from the first collected tube. The results by electrophoresis indicated that the components having antigen specific agglutination were mainly located on the B band of the electrophoretic gel. After being treated with 2 mercaptoethanol, the agglutination acvtivity for components dropped greatly. By comparison with non immunized control group, electrophoresis under non reducing conditions identified an extra B band with Mr=309 kD in the immunized samples. When this B band was isolated and subjected to electrophoresis under denaturing conditions, two separate polypeptides were detected with Mr of 52 kD and 26 kD, respectively, which should be identical of the molecular weights of fish IgG heavy and light chains. Therefore, we conclude that the A. hydrophila induced B bind likely represents a fish IgM class polymerized with four IgG molecules." @default.
- W2352198342 created "2016-06-24" @default.
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- W2352198342 date "2001-01-01" @default.
- W2352198342 modified "2023-09-28" @default.
- W2352198342 title "ANALYSIS ON IMMUNOGLOBULIN INDUCED BY INOCULATION OF AEROMONAS HYDROPHILA IN GRASS CARP (CTENOPHARYNGODEN IDELLUS CUVIER ET VALENCINNES)" @default.
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