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- W2352201364 abstract "Objective To construct the human hepatocyte model expressing HCV-C gene.Methods The HCV-C gene was amplified by PCR from the plasmid pHCV-MA,then it was cut with restriction enzymes and cloned into the plasmid pTRE to form recombinant plasmid pTRE-C.The plasmid pTRE-C was cut with restriction enzymes,and the aim fragment was ligated to the plasmid PcDNA3.The correct recombinant plasmid PcDNA3-tRe-C was screened out with analysis of restriction enzymes and PCR,then transferred into cultured human hepatocytes of Chang-liver by lipofectin,and established the cell model which expressed HCV-C gene.The HCV-C gene in Chang-liver hepatocytes was identified by PCR.The expression of HCV-C gene in Chang-liver hepatocytes was detected by RT-PCR.The expression and location of HCV core protein were identified by immunocytochemistry.Results The eukaryote expressing recombinant plasmid vector was constructed.Furthermore,the recombinant plasmid expressed the expected HCV-C protein in the human hepatocytes.Conclusion The human hepatocyte model expressing HCV-C gene was successfully established.This model was very useful for the future research of HCV-C biological characters and of mechanism of hepatocirrhosis and cancinogenesis resulting from HCV infection." @default.
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- W2352201364 date "2003-01-01" @default.
- W2352201364 modified "2023-09-24" @default.
- W2352201364 title "Establishment of human hepatocyte model expressing HCV-C gene" @default.
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