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- W2352207625 abstract "The adult rat hippocampal plasma membrane was purified by differential velocity centrifugation and continuous sucrose density-gradient centrifugation sequentially. Validation of the obtained plasma membrane was performed with Western blotting. The results showed that there were two plasma membrane bands within the gradient matrix, the major in the region of 32%～42% sucrose (density 1.13～1.18) and the minor in the region of 20%～25% sucrose (density 1.08～1.10); the region of about 39% sucrose (density 1.17) contained highest concentration and purity of plasma membrane. After analysis by SDS-PAGE and CapLC-MS/MS, totals of 135 proteins of rat hippocampal plasma membrane preparations were identified through database searching. Of the identified proteins, 70 were plasma membrane and membrane-associated proteins (accounting for 51.9 %), including Na+/K+-ATPase, Glutamate/aspartate transporter, Lipophilin, GLAST 1a and so on. The present experimental results accumulated valuable data for understanding the function of hippocampus and constructing the protein database specific for rat hippocampus." @default.
- W2352207625 created "2016-06-24" @default.
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- W2352207625 date "2006-01-01" @default.
- W2352207625 modified "2023-09-23" @default.
- W2352207625 title "Separation of Adult Rat Hippocampal Plasma Membrane and Its Proteomic Analysis" @default.
- W2352207625 hasPublicationYear "2006" @default.
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