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- W2352208088 abstract "Objective To choose proper carriers for expression of cPLA 2 cDNA and lay a foundation for further research on the effect of many factors on the activity of enzyme.Methods From cPLA 2 PMT 2 recombinants,human cPLA 2 cDNA fragments were taken and ligated to PRC/CMV,then mouse macrophages were transfected for screening.Positive cells were increased and RNA was extracted,finally cPLA 2 mRNA was detected with northern blot.Results With cPLA 2 PRC/CMV combinant transfecting marcrophages,cPLA 2 mRNA expression was detected by northern blot.Conclusion cPLA 2 PRC/CMV is an effective expression system." @default.
- W2352208088 created "2016-06-24" @default.
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- W2352208088 date "2001-01-01" @default.
- W2352208088 modified "2023-09-23" @default.
- W2352208088 title "Ligation and expression of cytosolic phospholipase A_2 cDNA and its carrier PRC/CMV" @default.
- W2352208088 hasPublicationYear "2001" @default.
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