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- W2352289317 abstract "In order to know the distribution of Fusarium tricinctum in Tianzhu Alpine Grassland in Gansu,China,rhizosphere soils around gramineous grass were sampled through a zigzag way from 16 sampling sites at 30 m interval at a elevation ranging between 2 880～3 360 m,5 sub-samples in total were picked up from each sampling site,and root debris from the soils was plated on Fusarium specific medium.The Fusarium colonies were purified and single-spored.Based on the morphological characteristics of Fusarium isolates,and according to the references related to Nelson’s Fusarium taxonomical system,212 Fusarium isolates were obtained and 5 of them were identified belonging to F.tricinctum.Two F.tricinctum isolates of the 5 were randomly selected for sequences analysis of β-tubulin gene.The PCR product of the two isolates were collected,purified,and sequenced.The sequences were aligned in GenBank.It was showed that there was a very close relationship of isolates TZh-4-3-7 and TZh-11-5-2 with the 6 F.tricinctum isolates in New Zealand,and their max similarity was 96%.Microsoft DNAStar was used to draw the phylogenetic tree of isolates TZh-4-3-7 and TZh-11-5-2.The tree showed that both TZh-4-3-7 and TZh-11-5-2 were in the same cluster as the 6 New Zealand isolates.The sequence analysis of β-tubulin gene determined the morphological identification of the 5 F.tricinctum isolates in this study,and the analysis was used to identify F.tricinctum for the first time in China.This study would enrich the future research on grassland degeneration associated with Fusarium." @default.
- W2352289317 created "2016-06-24" @default.
- W2352289317 creator A5057234288 @default.
- W2352289317 date "2011-01-01" @default.
- W2352289317 modified "2023-09-26" @default.
- W2352289317 title "Isolation,Identification and Analysis of β-tubulin Gene Sequence of Fusarium tricinctum at Different Altitudes in Tianzhu Alpine Grassland" @default.
- W2352289317 hasPublicationYear "2011" @default.
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