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- W2352391861 abstract "Objective: To explore high-level secretive expressed fusion protein of toxoplasma major surface antigen P30 with cholera toxin A2/B in Pichia pastoris. Methods: A compound gene fragment including P30 and cholera toxin A2/B was inserted into expression vector pGAPZαA by subclone technique , then the recombinants were transferred into yeast GS115 by electroporation and Pichia transformants were identified by PCR. The expression product was analyzed by means of SDS-PAGE and Western bolting. Results: The positive recombinant plasmids pMD18T-P30-CTA2/B and pGAPZαA-P30-CTA2/B were constructed successfully. A specific 49kDa fusion protein was expressed in Pichia pastoris, the product amounted to 570 mg/ L. The fusion protein P30-CTA2/B had good antigenicity. Conclusion: High-level expression of secreted P30-CTA2/B fusion protein is achieved in Pichia pastoris expression system." @default.
- W2352391861 created "2016-06-24" @default.
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- W2352391861 date "2005-01-01" @default.
- W2352391861 modified "2023-09-28" @default.
- W2352391861 title "Secretive expression of toxoplasma major surface antigen P30 fusion protein with cholera toxin A2/B in Pichia pastoris" @default.
- W2352391861 hasPublicationYear "2005" @default.
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