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- W2352488170 abstract "Objective:To explore the feasibility of the differentiation of rat bone marrow mesenchymal stem cell(BMSCs) into corneal stromal cells in vitro and the feasibility of fibrin glue(FG) as the scaffold of cell sheet engineering.Methods:BMSCs was obtained by density gradient centrifugation.The cells were divided into three groups:differentiation to keratocyte phenotype was induced in pellet cultures with serum-free;differentiation of BMSCs was induced in pellet cultures by co-cultured with corneal stromal cell;differentiation of BMSCs was induced in fibrin gels cultures by co-cultured with corneal stromal cell.Keratocyte-specific gene expression was characterized using western blotting.Morphological observations were performed with phpse contrast microscope.Results:BMSCs cultured in vitro showed great potential of proliferation.CD29 was positive,indicating that the cultured cells were MSC.Fibrin glue prepared was smooth and transparent with cell growth,after co-cultured with corneal strom al fibroblasts for one week,BMSCs expressed Keratocan,indicating that they had been transdifferentiated into corneal stromal cell.Conclusion:The BMSCs showed the tendency of induction from BMSCs into norneal stromal cell in exoteie-microenviroment.Fibrin glue was well compatible with corneal cells,which could con-struct tissue engineered cell with fibrin glue." @default.
- W2352488170 created "2016-06-24" @default.
- W2352488170 creator A5037058455 @default.
- W2352488170 date "2013-01-01" @default.
- W2352488170 modified "2023-09-23" @default.
- W2352488170 title "Study on the Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells on fibrin glue into corneal stromal Cells in Vitro" @default.
- W2352488170 hasPublicationYear "2013" @default.
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