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- W2352505846 abstract "Objective: To study the methods how to isolate and store cord blood nucleated cell(CBNC).Methods:Using three kind of methods(lymphocyte separating medium,methylcellulose,erythrocyte lysate) to isolate CBNC from cord blood,then freezing,storage and resuscitation with different storage liquid.Results: The method of erythrocyte lysate could collect far more nucleated cell than other two methods(P0.05 and P0.001) comparing with methods of lymphocytes separating medium and methylcellulose respectively that including lymphocyte,neutrophilic granulocyte and monocyte.In cell storage study,no difference of nucleated cells activity was found in H3-TdR during 24 hours between 4℃ and 20℃.It could be resuscitation and cultivation forming colony obviously even after one year store in liquid nitrogen canister with human serum from cord blood. Conclusion: Applying erythrocyte lysate to isolate karyocyte from cord blood and storage with human serum from cord blood is good way for CBNC collection,freezing and storage." @default.
- W2352505846 created "2016-06-24" @default.
- W2352505846 creator A5023482268 @default.
- W2352505846 date "2008-01-01" @default.
- W2352505846 modified "2023-09-22" @default.
- W2352505846 title "Study of isolation,freeze,thaw and storage of nuclear cells from cord blood" @default.
- W2352505846 hasPublicationYear "2008" @default.
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