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- W2352521928 abstract "[Objective] To obtain the HCC-associated antigens,a powerful new method,SEREX (serological identification of antigen by recombinant cDNA expression library),was used for identifying the antigens eliciting humoral immune response. Before performring serological analysis,a high quality cDNA library derived from human hepatocellular carcinoma (HCC) tissue was constructed. The primary library consisted of 3.96 ×106 recombinants and the recombinant rate was 93%. For better preserving the cDNA library,it was amplified. As a result,the titer of the amplified cDNA library was 3.92×109 pfu/ml. With SEREX method,immunoscreening for the detection of reactive clones in the human NPC tissue cDNA library was performed with autologous serum. As a result,31positive clones encoding antigenic genes were obtained after immunoscreening,and the nucleotide sequences of cDNA inserts were determined and analyzed with BLAST software in GenBank. Results showed that the 31 reactive clones were derived from 24 different genes. 17 of 24 genes had high homologous to the genes known in GenBank,However,there were also 7 genes with low homology to the genes known in GenBank. Furthermore,3 of 7 genes may be novel genes. The associations of these genes to HCC and the roles that they played in the occurrence and development of HCC should be revealed by further research." @default.
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- W2352521928 date "2008-01-01" @default.
- W2352521928 modified "2023-09-24" @default.
- W2352521928 title "Construction of cDNA library from hepatocellular carcinoma tissue and screening of antigenic genes" @default.
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