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- W2352563561 abstract "Objective To generate a cell line overexpressing SOCS1 protein and to investigate the protective effect of SOCS-1 on apoptosis of pancreatic β cell.Methods The rat SOCS-1 coding sequence was amplified and subcloned into pEGFP-C1 vector.The recombinant plasmid pEGFP-C1-SOCS1 was transfected into RINm5F cell line(insulin-secreting tumor cell line).We generated a rat RINm5F cell line overexpressing recombinant plasmid pEGFP-C1-SOCS1.Cells were treated by several cytokines,including TNF-α,IL-1β,IFN-γ,IL-1β+IFN-γ,IL-1β+TNF-α+IFN-γ.The cellular apoptosis were measured by RT-PCR and Western blot.Results We have generated a rat RINm5F cell line overexpressing SOCS1 protein successfully.The expression level of the iNOS mRNA was significantly increased in RINm5F cells after treatment by IFN-γ,IL-1β+IFN-γ,IL-1β+TNF-α+IFN-γ,but notSOCS1-RINm5F cells.The expression and activity of caspase-3 was significantly increased in RINm5F cells after treatment by all cytokines.The caspase-3 expression and activity were not significantly changed in SOCS1-RINm5F cells after treated by cytokines.Conclusion Overexpression of SOCS-1 can reduce cytokine-induced pro-apoptosis gene expression in pancreatic β cells." @default.
- W2352563561 created "2016-06-24" @default.
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- W2352563561 date "2010-01-01" @default.
- W2352563561 modified "2023-09-25" @default.
- W2352563561 title "SOCS-1 reduced the cytokine-induced pro-apoptosis gene expression in pancreatic β cells" @default.
- W2352563561 hasPublicationYear "2010" @default.
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