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- W2352579197 abstract "Objective To construct human parvovirus B19 VP1 gene prokaryotic expression clones. Methods VP1 gene, the main antigenic determinants of B19 virus, was amplified by PCR technique and inserted into the prokaryotic expression plasmid pGEX 4T 2, the expressed fusion protein was induced with IPTG and purified by affinity chromatography and its antigenicity was identified by Western blot, also the ELISA coating with the recombinant protein was used to screen the clinical serums. Results pGEX 4T 2 VP1 plasmid expressed a fusion protein efficiently in E. coli., ELISA analysis showed that the purified expressed fusion protein had a good agreement with the Virion ELISA kit. Conclusion This expressed protein had strong antigenic characterization, it will provide valuable information for developing B19 recombinant antigen ELISA kit in our country." @default.
- W2352579197 created "2016-06-24" @default.
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- W2352579197 date "2002-01-01" @default.
- W2352579197 modified "2023-09-23" @default.
- W2352579197 title "CONSTRUCTION OF HUMAN PARVOVIRUS B19 VP1 GENE PROKARYOTIC EXPRESSION CLONES" @default.
- W2352579197 hasPublicationYear "2002" @default.
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