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- W2352579432 abstract "A tet-on regulating system of the functional Runx2 expression was established in mouse myoblast cell line C2C12.A pTet-on regulating plasmid was first transfected into C2C12 and selected with G418 for stable clones.Then the pTRE-Flag-Runx2 was transfected into the obtained positive C2C12/Tet cells and selected with hygromycin.When Dox was used to induce the expression of Runx2,a sensitive clone was screened through quantitative RT-PCR.The optimal Dox induction condition of 10 μg/ml for 12 hours was determined by quantitative RT-PCR and Western blot assays.ALP staining and Alizarin red(AZR) staining after Dox treatments were used to evaluate the capability of osteogenic differentiation.The levels of differentiation of C2C12/Tet/pTRE-Flag-Runx2 cells were significantly higher than those of native C2C12 cells or C2C12/Tet/pTRE cells(P0.05 or 0.01).Our Tet-on regulated Runx2 expression system in C2C12 cells may provide a useful tool for the further study of Runx2 functions." @default.
- W2352579432 created "2016-06-24" @default.
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- W2352579432 date "2010-01-01" @default.
- W2352579432 modified "2023-09-23" @default.
- W2352579432 title "Overexpression of Runx2 Induces Osteogenic Differentiation in C2C12 Cells" @default.
- W2352579432 hasPublicationYear "2010" @default.
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