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- W2352672119 abstract "The V H and V L genes were amplified from two hybridoma cell lines producing mouse McAb against alpha txoin of Clostridium perfringens type A by RT PCR.The V H and V L genes were connected through a flexible Linker (Gly 4Ser) 3,and the V H Linker V L (ScFv) fusion gene was cloned into a clone vector pGEM T.The 2E3 ScFv and 1A8 ScFv gene were sequenced and analyzed by computer.The ScFv genes consist of 726bp and 729bp respectively.Both V H and V L genes were functionally rearranged mouse immunoglobulin variable region genes and appeared to be new genes.According to Kabat classed method,the V H gene segment and V L gene segment of 2E3 ScFv belong to the mouse Ig heavy chain subgroupⅡ(B) and κ chain subgroup Ⅲ respectively,but the V H gene segment and V L gene segment of 1A8 ScFv belong to the mouse Ig heavy chain subgroup Ⅱ(A) and κ chain subgroup Ⅵ respectively." @default.
- W2352672119 created "2016-06-24" @default.
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- W2352672119 date "2001-01-01" @default.
- W2352672119 modified "2023-09-25" @default.
- W2352672119 title "Sequence Analysis of ScFv Gene for Anti-alpha-toxin" @default.
- W2352672119 hasPublicationYear "2001" @default.
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