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- W2352672783 abstract "An analytical method based on HPLC-DAD has been developed for the determination of methotrexate in cosmetics.Various cosmetic samples,including lotion,cream,emollient,waterpowder and lipstick,were extracted under ultrasonication.The filtrate was injected into HPLC and detected at 302 nm.Qualitative analysis of methotrexate was based onthe retention time of the chromatogram and ultraviolet spectrum.Quantitative analysis was conducted by external standard methods.The calibration curves of the methotrexate was linear(r=0.9999) between 0.05 and 100 mg/L.The recoveries were from 87.8% to 103.4% with relative standard deviations of 0.42% to 6.8%.The limit of detection was 0.02 mg/L and the limit of quantitation was 0.05 mg/L.The method is simple and rapid with high accuracy,and suitable for the determination of methotrexate in cosmetics." @default.
- W2352672783 created "2016-06-24" @default.
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- W2352672783 date "2009-01-01" @default.
- W2352672783 modified "2023-09-25" @default.
- W2352672783 title "Determination of methotrexate in cosmetics by HPLC/DAD" @default.
- W2352672783 hasPublicationYear "2009" @default.
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