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- W2352707029 abstract "Objective To investigate genotypes of extended-spectrumβ-lactamases(ESBLs)produced by Escherichia coli(E.coli)and Klebsiella pneumoniae(K.pneumoniae)isolated from a hospital.Methods Clinical isolates of ESBLs-producing E.coli(n=40)and K.pneumoniae(n=20)were collected according to ESBLs confirmatory test.Plasmid DNA was extracted by alkaline lysis method.PCR analysis was used to amplify TEM,SHV,and CTX-M genes,sequence analysis was performed to identifyβ-lactamase genotype.Results Sixty confirmatory test positive E.coli and K.pneumoniae were positive for PCR amplification,including TEM,SHV,CTX-M-3 and CTX-M-9.The positive rate of above four genotypes among E.coli was 37.50%(15 isolates),2.50%(1 isolate),62.50%(25 isolates)and 50.00%(20 isolates)respectively;the positive rate of above four genotypes among K.pneumoniae was 40.00%(8 isolates),90.00%(18 isolates),65.00%(13 isolates)and 40.00%(8 isolates)respectively.100.00% of E.coli and 80.00% of K.pneumoniae expressed blaCTX-M,12.50%(5/40)of E.coli and25.00%(5/20)of K.pneumoniae carried two types of CTX-M gene.Twenty-three isolates with TEM gene were TEM-1;nineteen isolates with SHV gene included 6isolates of genotype SHV-1,6 of SHV-11,5 of SHV-12 and 2of SHV-25,only SHV-12 was ESBLs gene,all these types were fromK.pneumoniae;66isolates were with CTX-M gene,the detection rate of CTX-M-14 among E.coli and K.pneumoniae was 45.00% and 35.00% respectively,CTX-M-55 were both35.00%,CTX-M-15 was 20.00%and 15.00%respectively,CTX-M-3,CTX-M-65,CTX-M-101 and CTX-M-123 genotypes accounted for fewer percentage of CTX-M genotype.Conclusion The majority of ESBL gene in E.coli and K.pneumoniaein this hospital is CTX-M,the next is SHV-12.The most prevalent ESBL genotype is CTX-M-14.CTX-M-101 and CTX-M-123 are initially detected in Shandong province." @default.
- W2352707029 created "2016-06-24" @default.
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- W2352707029 date "2013-01-01" @default.
- W2352707029 modified "2023-09-23" @default.
- W2352707029 title "Detection and genotyping of extended-spectrumβ-lactamases in Escherichia coli and Klebsiella pneumoniae isolated from a hospital" @default.
- W2352707029 hasPublicationYear "2013" @default.
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