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- W2352734890 abstract "Objective: To compare explant techniques and monolayer culture in culturing human periodontal ligament fibroblasts. Methods: Cell cultures were performed from human periodontal ligament by means of explant techniques and monolayer culture. Cells were characterized on the basis of morphological, biochemical, and proliferative parameters. Result: HPLFs cultured in two different methods were similar in maphology and proliferation. Cells cultured in explant techniques needed a long period of time to confluent in the primary culture; Cells obtained from monolayer culture were mixed with epithelial cells and must be purified by several processes of passive culture. Conclusion: Explant techniques used to culture HPLFs can help us obtain comparatively pure cells, but the primary culture needs a long period of time. Cells obtained from monolayer culture are more than from explant techniques in a short period of time, but we have to purify cells by successive passage culture." @default.
- W2352734890 created "2016-06-24" @default.
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- W2352734890 date "2002-01-01" @default.
- W2352734890 modified "2023-09-25" @default.
- W2352734890 title "Explant techniques and monolayer culture in culturing human periodontal ligament fibroblasts" @default.
- W2352734890 hasPublicationYear "2002" @default.
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