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- W2352804992 abstract "Objective: To establish the determination method for polysorbate 80 in Fufang kushen injections by HPLC-ELSD,compare it with UV spectrophotometry. Method: HPLC-ELSD was adopted with a TSKgeL G2000SWXL( 7. 8 mm × 300 mm,5 μm) column. The mobile phase consisted of 0. 02 mol·L- 1ammonium acetate-acetonitrile( 88 ∶ 12) with the flow rate of 0. 6 mL·min- 1and the column temperature was30 ℃. The temperature of drift tube was 80 ℃. The flow rate of N2was 2 L·min- 1. Result: The linear range of polysorbate 80 was13. 11-52. 42 μg( r = 0. 999 2),and the average recovery was 100. 9%,RSD 2. 2%. Compared with the theoretical value of Polysorbate 80 in Fufang kushen injections,result of UV on the high side,results of HPLC-ELSD method was closer to the theoretical value. Conclusion: HPLC-ELSD is simple and accurate,and can be used in the determination of polysorbate 80." @default.
- W2352804992 created "2016-06-24" @default.
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- W2352804992 date "2014-01-01" @default.
- W2352804992 modified "2023-09-28" @default.
- W2352804992 title "Two Methods for Determination of Polysorbate 80 in Fufang Kushen Injections" @default.
- W2352804992 hasPublicationYear "2014" @default.
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