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- W2352808701 abstract "Objective To investigate endogenic nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) expression in herpes simplex type-1-virus (HSV-1) -infected neuroglia cells. Methods U251 human glioma cells were cultured in RPMI-1640 medium and infected with HSV-1 in vitro to establish a cell model of viral infection. Establishment of HSV-1-infected U251 cells was identified by HSV-1 gD mRNA expression. NGF and BDNF expression was detected by RT-PCR and Western blotting, respectively, in normal U251 cells and HSV-1-infected U251 cells. Results HSV-1 could efficiently infect U251 cells. NGF and BDNF was identified to be expressed in U251. NGF and BDNF expression reached a maximum at 6 hours post HSV-1 infection, then NGF and BDNF expression decreased in time-dependent manner. Conclusions HSV-1 infection could dysregulate NGF and BDNF expression in the U251 cells." @default.
- W2352808701 created "2016-06-24" @default.
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- W2352808701 date "2011-01-01" @default.
- W2352808701 modified "2023-09-26" @default.
- W2352808701 title "Study of NGF and BDNF expression in HSV-1 infected human glioma cells" @default.
- W2352808701 hasPublicationYear "2011" @default.
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