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- W2352892747 abstract "Objective To study the transfer and expression of firefly lucificerase mediated by cationic liposome in four cell lines.Methods Plasmid pDR 2luc were amplified in E.coli DH 5a strain cells,extracted by alkaline lysis and purified by passage through Sepharose 2B column chromatography.Four cell lines including human hepatocarcinoma cell line HepG 2 and SMMC7721,human renal carcinoma cell line GRC and simian kidney cell line COS 7 were be transfected by cationic liposome Lipofectin.The activity of firefly lucifierase gene were detected with scintillation count.Results The activity of firefly lucificerase gene were detected in four cell lines significantly( P 0 05).Conclusion Cationic liposome Lipofectin is a kind of cytofectin capable of transferring DNA into various cells." @default.
- W2352892747 created "2016-06-24" @default.
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- W2352892747 date "2004-01-01" @default.
- W2352892747 modified "2023-09-22" @default.
- W2352892747 title "Cationic liposome mediated-transter and expression of firelfy luciferase gene" @default.
- W2352892747 hasPublicationYear "2004" @default.
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