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- W2352904168 abstract "In spite of the presence of ubiquitous DNase in the environment, high-molecular-weight extracellular DNA molecules can survive in natural habitats such as soil and maintain the transforming ability. It has been evidenced that DNA molecules, once being absorbed in sand, can be protected from DNase degradation. However, the mechanism of DNA-sand interaction and its effect on transforming ability remain unclear. In the present study, we developed a flow-through system of sand-filled micro Bio-Spin columns to determine the distribution of extracellular DNA in sand and employed E.coli transformation method to directly monitor its transforming ability. 20 μL of free plasmid pUC18 DNA was introduced into 1.0 g sand (1.2mm diameter 2.5mm), eluted by 1.5 mL of 0.1 mol/L CaCl_2 and extracted by 200 μL of TEN buffer. The transforming ability and stability of the plasmid DNA in the eluate, extract and sand were examined with three different transformation protocols. The results indicated that the DNA transforming ability and accessibility to DNaseⅠ in eluate, extract and sand were different, suggesting at least three types of DNA-sand complexes. Moreover, the transforming ability of adsorbed DNA in sand was detected over a period of 35 days, whereas the activity of free plasmid without protection of sand only maintained for 7 days. In the presence of competent cells, the DNA molecules, released from other organisms, were uptaken by the competent cells insteadof being absorbed by sand. This result suggested that the transforming ability of sand adsorbed-DNA was affected by the conformation of DNA molecules and the accessibility to competent cells, but not the DNA concentration. Our data showed that there were at least three forms of DNA in the environment, which might make different contributions to the dynamic change of extracellular DNA pool." @default.
- W2352904168 created "2016-06-24" @default.
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- W2352904168 date "2005-01-01" @default.
- W2352904168 modified "2023-09-25" @default.
- W2352904168 title "The distribution and transforming ability of sand-absorbed DNA" @default.
- W2352904168 hasPublicationYear "2005" @default.
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