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- W2353063701 abstract "The open reading frame (ORF) 1 and 2 gene of porcine circovirus (PCV) serotype 2 were amplified by PCR and cloned into the shuttle plasmid of pAdTrack-CMV. The recombinant adenovirus genomic DNA was obtained by cotransformation of the recombinant plasmid and adenovirus backbone DNA into E.coli BJ5183, which was then used to transfect 293 cell line by the lipofectamine method. Recombinant adenovirus produced from the cells was used to inoculate 6 to 8 weeks BALB/c mice by nasal drip two times. Immune sera were obtained through the tails of the mice. The serum antibody against PCV2 was detected by ELISA, and the results showed that the recombinant adenovirus could induce the specific antibody against PCV2." @default.
- W2353063701 created "2016-06-24" @default.
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- W2353063701 date "2008-01-01" @default.
- W2353063701 modified "2023-09-28" @default.
- W2353063701 title "Construction of recombinant adenovirus carrying ORF1, ORF2 gene of porcine circovirus2" @default.
- W2353063701 hasPublicationYear "2008" @default.
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