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- W2353076665 abstract "In the present study,the ORF5M gene(the modified ORF5 gene) and ORF6 gene,the two major immunogenicity genes of PRRSV,were used as the target genes,and bovine herpesvirus 1(BHV-1)VP22 possessing protein transduction property was chosed as the immunoadjuvant.Based on homologous recombination,a recombinant PRV(rPRV) TK~-/gE~-/VP22E~+/VP22M~+ coexpressing PRRSV E and M protein fused with BHV-1 VP22 protein was constructed by using PRV TK~-/gE~-/LacZ~+ as parent strain.The rPRV was confirmed by PCR,Southern blot and Western blot.The results of TCID_(50) tests showed that the insertion of the foreign genes had no influence on the propagation of rPRV in IBRS-2 or PK15 cells.BALB/c mice were immunized with rPRV TK~-/gE~-/VP22E~+/VP22M~+,and compared with rPRV TK~-/gE~-/E~+ expressing ORF5M gene and TK~-/gE~-/E~+/M~+ coexpressing ORF5M and ORF6 genes respectively.Neutralizing antibodies and lymphocyte proliferative responses were evaluated at 10 weeks after primary immunization.Among three rPRVs,TK~-/gE~-/VP22E~+/VP22M~+ had the best effect on enhancing PRRSV-specific humoral and cellular immune responses,and VP22 showed adjuvant efficiencies on rPRV vaccine to a certain degree.In conclusion,the study established a base for the research of bivalent genetic engineering vaccines against PRRSV and PRV." @default.
- W2353076665 created "2016-06-24" @default.
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- W2353076665 date "2006-01-01" @default.
- W2353076665 modified "2023-09-28" @default.
- W2353076665 title "Construction of Recombinant Pseudorabies Virus Coexpressing E and M Protein of Porcine Reproductive and Respiratory Syndrome Virus Fused with VP22 Protein of Bovine Herpesvirus 1 and Its Immunogenicity in Mice" @default.
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