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- W2353098086 abstract "Constructing a rice cDNA library is a fundamental basis for understanding molecular interactions of rice with Xanthomonas oryzae pv.oryzicola,the critical pathogen of bacterial leaf streak in rice.The total RNA were extracted from rice leaves previously infected for 10 hours by the pathogen.The cDNA were reversely transcripted from the mRNA using SMARTTM technique and then ligated into a vector pGADT7-SfiAB at the sites of SfiA and SfiB.Collections of the ligated plasmid DNA were the cDNA library of rice which had interactions with the pathogen.The PCR amplification of OsPR-1a,OsPR-1b and PAL as pathogenesis-related protein genes indicated that the cDNA library contained those genes.Digestion of randomly selected clones of the library with the enzyme SfiⅠ demonstrated that the sizes of inserted fragments in the vector were between 500-2 000 bp,that the average size was about 1 kb,and that 95% of the library clones were recombinants.The above suggests that the constructed cDNA library of rice is of good quality and of use for isolation of interacting proteins with pathogenicity determinants of the bacterium by a yeast two-hybrid system." @default.
- W2353098086 created "2016-06-24" @default.
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- W2353098086 date "2009-01-01" @default.
- W2353098086 modified "2023-09-23" @default.
- W2353098086 title "Construction of a rice cDNA library induced by infection of Xanthomonas oryzae pv.oryzicola" @default.
- W2353098086 hasPublicationYear "2009" @default.
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