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- W2353105280 abstract "Based on the regeneration system established,the Cucumis sativus cv.inbred line M8 which has strong capability of regeneration was used as experiment material to transform invertase inhibitor INH gene into cucumber by using Agrobacterium tumefaciens-mediated gene transfer.Cotyledon explants were immersed in an Agrobacterium suspension and inoculated in the select medium that was on the concentration gradient of 1 mg.L-1 to 10 mg.L-1 to study the resistance and sensitivity of the cotyledon explants to hygromycin.The effect of immersing time on the conversion efficiency for 5 min,10 min,15 min,20 min with Agrobacterium suspension was studid.The results showed that the highest regeneration frequency was 18.9% when the choice of the HygB pressure levels was 6 mg.L-1 and the infection time was 15 min.The PCR analysis showed that the conversion of INT gene had been integrated into the cucumber genome with the conversion rate of 2.0%." @default.
- W2353105280 created "2016-06-24" @default.
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- W2353105280 date "2013-01-01" @default.
- W2353105280 modified "2023-09-26" @default.
- W2353105280 title "Effect of Hygromycin Concentration and Agrobacterium Immersing Time on Cucumber (Cucumis Sativus L.) Explant Regeneration" @default.
- W2353105280 hasPublicationYear "2013" @default.
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