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- W2353124868 abstract "Objective To investigate the effect of inhibition of VEGF on immune function of dendritic cell(DC) secreted by MCF-7 breast cancer cells. Methods siRNA-VEGF was constructed and transfected into MCF-7 breast cancer cell by using cationic liposome Lipofectamine 2000TM as transfecting agent,the changes of VEGF gene expression were measured by reverse transcription-polymerase chain reaction(RT-PCR),and Western blotting in vitro,the VEGF silencing result was observed.Mononuclear cells were cultured in the culture supernatants from fresh primary MCF-7 cells(control group) and from siRNA-VEGF treated MCF-7 cells(siRNA-VEGF group) with granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin 4(IL-4) and tumor necrosis factor-α(TNF-α).The proliferative ability of T cells from healthy volunteers stimulated by DCs and the tumoricidal activity of CTL primed by DCs were measured by MTT assay and Hoechst33258.The concentrations of IL-12 in the supernatant of cultured DCs and IFN-γ in the supernatant of cocultivated DCs periphra1 blood mononuclear cells(PBMC) were detected by ELISA. Results siRNA directed against VEGF gene effectively down-regulated the expression of VEGF in the level of mRNA and protein.The tumoricidal activity against MCF-7 breast cancer cells,and the concentration of IL-12 secreted by DCs and IFN-γ produced by PBMC were all significantly higher than that in the control group. Conclusion siRNA can aim at VEGF secreted by MCF-7 cells to inhibit its expression,the inhibition of the supernatant from MCF-7 cells on DCs decreased markedly after VEGF gene was down-regulated by siRNA." @default.
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- W2353124868 date "2009-01-01" @default.
- W2353124868 modified "2023-09-25" @default.
- W2353124868 title "THE INFLUENCE OF SUPPRESSION OF VEGF EXPRESSION SECRETED BY MCF-7 BREAST CANCER CELL ON IMMUNE FUNCTION OF DENDRITIC CELL" @default.
- W2353124868 hasPublicationYear "2009" @default.
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