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- W2353170899 abstract "It has been confirmed that hemocyanin is a kind of important multifunctional protein found in the hemolymph of both arthropod and mollusk. And phenoloxidase is a type of vital immune protein in invertebrate. Recently,interest has been focused on comparative research between hemocyanin and phenoloxidase. In the paper,a preliminary study was made to characterize the variation of phenoloxidase activity affected by in vivo hemocyanin and in vitro hemocyanin recombinant protein in shrimp Penaeus vannamei obtained from Xiamen. The methods of artificial infection by V. parahaemolyticus and measurement of both concentration of hemocyanin and phenoloxidase activity were used. The results showed that the variational trend of hemocyanin's concentration was similar to that of phenoloxidase activity after the shrimp was infected by V. parahaemolyticus,both of which increased to their maximum in 24?h,then descended slowly,finally came back to normal level in 36?h. In comparison with control group in vitro, the shrimp serum's phenoloxidase activity increased obviously after hemocyanin recombinant protein was added in at the concentration of 200?μg/mL or ultrasonic solution of E. coli K12 at the concentration of 35?mg/mL. However,when both hemocyanin recombinant protein and ultrasonic solution of E. coli K12 were added into the shrimp serum, its phenoloxidase activity descended immediately. It suggests that hemocyanin can adjust the phenoloxidase activity by means of positive feedback,but it also can adjust the phenoloxidase activity by means of negative feedback when its reactive solution is involved in some bacteria. In summary,these results are the latest discovery in the field of the immune function of hemocyanin and phenoloxidase,which will be helpful for elucidating the immune mechanism of hemocyanin and interrelation between hemocyanin and phenoloxidase." @default.
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- W2353170899 date "2005-01-01" @default.
- W2353170899 modified "2023-09-25" @default.
- W2353170899 title "Variation of phenoloxidase activity affected by hemocyanin in shrimp Penaeus vannamei" @default.
- W2353170899 hasPublicationYear "2005" @default.
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