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- W2353213294 abstract "The objective of the present study was to identify horizontal transfer of gene fragment(T1C4) from E.coli to Shigella and its relationship with multi-drug resistance.Screened positive clone which contains T1C4 gene fragment was cultured and the plasmid was extracted.The fragment of T1C4 gene was then amplified by PCR and purified by gel purification kit.The probe was prepared and the dot hybridization was conducted for genomic DNA and plasmid DNA of Shigella sensitive strain(SS23),transferred multi-drug resistant Shigella strain(ST11) and Escherichia colimulti-drug resistant clinical strain(E667).Finally,antimicrobial sensitivity of DH5α,DH5α(pUC18),DH5α(pUC18-T1C4) and ATCC25922 were detected by disk diffusion testing.The procedure was carried out in accordance with the guidelines in standard of antibiotics susceptibility test(Kirby-Bauer method) document.Results displayed that T1C4 gene fragment was amplified from genomic DNA of ST11 and E667 by PCR.The genomic DNAs and plasmid DNAs of transferred strain(ST11) and clinical strain(E667) all gave positive signals in dot hybridization,whereas both of genomic DNA and plasmid DNA of SS23 gave negative hybridization signal.The bacteriostatic diameters of Tetracycline,Cefazolin,Cefalotin,Norfloxacin and SMZ-TMP to DH5α(pUC18-T1C4) were significantly shorter than those to DH5α(pUC18).It's concluded that gene fragment(T1C4) was transferred from E667 to ST11 with plasmid.Moreover,T1C4 gene fragment might be closely related to multi-drug resistance of bacteria." @default.
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- W2353213294 date "2010-01-01" @default.
- W2353213294 modified "2023-09-26" @default.
- W2353213294 title "Identification on horizontal transfer of multi-drug resistance related gene fragment of Shigella and its drug-resistance" @default.
- W2353213294 hasPublicationYear "2010" @default.
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