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- W2353234564 abstract "Objective To develop a double monoclonal antibodies sandwhich ELISA assay for the detection EPF. Methods The hybridoma cell lines that secrete monoclonal antibodies against EPF were screened. Ascitic fluid containing the monoclonal antibodies against EPF was obtained by intraperitoneal injection of the hybridoma cells. Purified monoclonal antibodies were labeled with HRP by sodium iodide method. Capture and sandwich McAbs were selected by sandwich ELISA matched with each other. A double EPF-McAb sandwich ELISA method for measurement EPF activity was then established. Results Two hybridoma cell lines that secrete monoclonal antibodies against EPF were obtained. The lowest threshold of detection was 0.01ug/ml, and the calibration curves for EPF were linear ranged between 0.01-10.0ug/mL, when two serum plasma samples from one batch were assayed repeatedly for 8 times and another 2 samples from other batch were assayed repeatedly for 6 times ,the average intra-assay and the inter-assay CV were 7.7% and 5.8% respectively. The sensitivity and specificity of this method were 90.2% and 92.8%. Conclusion This method is simple and rapid with high sensitivity and stability,which is valid for measurement of the serum EPF activity." @default.
- W2353234564 created "2016-06-24" @default.
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- W2353234564 date "2005-01-01" @default.
- W2353234564 modified "2023-09-26" @default.
- W2353234564 title "Establishment of EPF -McAb-ELISA in Measurement of EPF activity" @default.
- W2353234564 hasPublicationYear "2005" @default.
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