Matches in SemOpenAlex for { <https://semopenalex.org/work/W2353285212> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2353285212 abstract "In order to gain the main antigen domain of the classical swine fever virus(CSFV)E2protein,to establish rapid detection method for CSFV antigen and antibody.The main antigenic domain of CSFV E2 was amplified and subcoloned into prokaryotic expression plasmid pET-30a(+)vector.The recombinant plasmid named pET-30a-E2-1was transformed into E.coli Rosetta(DE3)induced by IPTG.Recombinant expression products were analyzed by SDS-PAGE,a specific expression band in form of inclusion body with a relative molecular weight 31.0ku was detected.The optimum temperature was 18℃,the IPTG was 0.1mmol/L.The expression amount accountsed for about 46% of the yield in total cell lysate.Western blot indicated that the expressed proteins were recognized not only by antibody against the C-strain of CSFV,but also by the specific monoclonal antibody of HIS tag.The result suggests that the recombinant protein has good antigenicity and provides substantial base for the CSFV antibody and antigen detection kit research." @default.
- W2353285212 created "2016-06-24" @default.
- W2353285212 creator A5079475024 @default.
- W2353285212 date "2013-01-01" @default.
- W2353285212 modified "2023-09-25" @default.
- W2353285212 title "Prokaryotic Expression and Identification of the Main Antigenic Domain in E2 Glycoprotein of Classical Swine Fever Virus" @default.
- W2353285212 hasPublicationYear "2013" @default.
- W2353285212 type Work @default.
- W2353285212 sameAs 2353285212 @default.
- W2353285212 citedByCount "0" @default.
- W2353285212 crossrefType "journal-article" @default.
- W2353285212 hasAuthorship W2353285212A5079475024 @default.
- W2353285212 hasConcept C104317684 @default.
- W2353285212 hasConcept C108625454 @default.
- W2353285212 hasConcept C147483822 @default.
- W2353285212 hasConcept C153911025 @default.
- W2353285212 hasConcept C159047783 @default.
- W2353285212 hasConcept C159654299 @default.
- W2353285212 hasConcept C170344550 @default.
- W2353285212 hasConcept C203014093 @default.
- W2353285212 hasConcept C22744801 @default.
- W2353285212 hasConcept C2522874641 @default.
- W2353285212 hasConcept C2776415932 @default.
- W2353285212 hasConcept C2779005923 @default.
- W2353285212 hasConcept C2779026604 @default.
- W2353285212 hasConcept C33161422 @default.
- W2353285212 hasConcept C40767141 @default.
- W2353285212 hasConcept C542903549 @default.
- W2353285212 hasConcept C54355233 @default.
- W2353285212 hasConcept C86803240 @default.
- W2353285212 hasConceptScore W2353285212C104317684 @default.
- W2353285212 hasConceptScore W2353285212C108625454 @default.
- W2353285212 hasConceptScore W2353285212C147483822 @default.
- W2353285212 hasConceptScore W2353285212C153911025 @default.
- W2353285212 hasConceptScore W2353285212C159047783 @default.
- W2353285212 hasConceptScore W2353285212C159654299 @default.
- W2353285212 hasConceptScore W2353285212C170344550 @default.
- W2353285212 hasConceptScore W2353285212C203014093 @default.
- W2353285212 hasConceptScore W2353285212C22744801 @default.
- W2353285212 hasConceptScore W2353285212C2522874641 @default.
- W2353285212 hasConceptScore W2353285212C2776415932 @default.
- W2353285212 hasConceptScore W2353285212C2779005923 @default.
- W2353285212 hasConceptScore W2353285212C2779026604 @default.
- W2353285212 hasConceptScore W2353285212C33161422 @default.
- W2353285212 hasConceptScore W2353285212C40767141 @default.
- W2353285212 hasConceptScore W2353285212C542903549 @default.
- W2353285212 hasConceptScore W2353285212C54355233 @default.
- W2353285212 hasConceptScore W2353285212C86803240 @default.
- W2353285212 hasLocation W23532852121 @default.
- W2353285212 hasOpenAccess W2353285212 @default.
- W2353285212 hasPrimaryLocation W23532852121 @default.
- W2353285212 hasRelatedWork W1856175899 @default.
- W2353285212 hasRelatedWork W2010796783 @default.
- W2353285212 hasRelatedWork W2042853577 @default.
- W2353285212 hasRelatedWork W205392829 @default.
- W2353285212 hasRelatedWork W2128722996 @default.
- W2353285212 hasRelatedWork W2285615348 @default.
- W2353285212 hasRelatedWork W2352116353 @default.
- W2353285212 hasRelatedWork W2369697688 @default.
- W2353285212 hasRelatedWork W2387358509 @default.
- W2353285212 hasRelatedWork W2409997549 @default.
- W2353285212 hasRelatedWork W2411956443 @default.
- W2353285212 hasRelatedWork W2751730111 @default.
- W2353285212 hasRelatedWork W2801050041 @default.
- W2353285212 hasRelatedWork W3031829574 @default.
- W2353285212 hasRelatedWork W3149558173 @default.
- W2353285212 hasRelatedWork W2815608108 @default.
- W2353285212 hasRelatedWork W2817286703 @default.
- W2353285212 hasRelatedWork W2862961082 @default.
- W2353285212 hasRelatedWork W2882298644 @default.
- W2353285212 hasRelatedWork W3142254843 @default.
- W2353285212 isParatext "false" @default.
- W2353285212 isRetracted "false" @default.
- W2353285212 magId "2353285212" @default.
- W2353285212 workType "article" @default.