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- W2353292502 abstract "OBJECTIVE To establish a coculture and a sequential system for human early embryo culture. METHODS The endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours. RESULTS Human endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%. CONCLUSION The autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality." @default.
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- W2353292502 date "2006-11-01" @default.
- W2353292502 modified "2023-09-22" @default.
- W2353292502 title "Establishment of autologous endometrial coculture and sequential system for human early embryo culture" @default.
- W2353292502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17146925" @default.
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