Matches in SemOpenAlex for { <https://semopenalex.org/work/W2353326121> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2353326121 abstract "[Objective] The purpose of this reseach was to clone the cDNA fragment of ACC synthase(1-aminocyclopropane-1-carboxylate synthase,ACS)of phalaenopsis and to construct its antisense expression vector.[Method] A pair of specific primers were designed and synthesized according to the reported cDNA sequences of phalanopsis ACC synthase gene(GenBank:Z77854.1;AF004663.1).The partial cDNA of ACS was obtained from total RNA of phalaenopsis by RT-PCR.Then the cDNA fragment was linked to T-tailing pMD19-T vector for cloning and sequencing.After the recombinant plasmid and plant expression vector pBI221 both being digested with Sac I and Xba I,the fragment of ACS was ligated to the CaMV35S promoter downstream of pBI221 in antisense orientation to construct the antisense expression vector.[Result] The cDNA fragment of phalaenopsis ACS was cloned.The sequencing results showed that the obtained sequence was made up of 461 bp and the homologous rate was 98.92% and 76.36% compared with reported sequences.Using appropriated site of restriction endonuclease,the antisense expression vector of phalaenopsis ACS was successfully constructed.[Conclusion] The antisense plant expression vector of phalaenopsis ACS was constructed,which laid foundation for further study of the effects on florescene and growth of phalanopsis." @default.
- W2353326121 created "2016-06-24" @default.
- W2353326121 creator A5048358604 @default.
- W2353326121 date "2009-01-01" @default.
- W2353326121 modified "2023-09-28" @default.
- W2353326121 title "cDNA Cloning and Construction of Antisense Expression Vector of Phalaenopsis ACC Synthase" @default.
- W2353326121 hasPublicationYear "2009" @default.
- W2353326121 type Work @default.
- W2353326121 sameAs 2353326121 @default.
- W2353326121 citedByCount "0" @default.
- W2353326121 crossrefType "journal-article" @default.
- W2353326121 hasAuthorship W2353326121A5048358604 @default.
- W2353326121 hasConcept C104317684 @default.
- W2353326121 hasConcept C121050878 @default.
- W2353326121 hasConcept C128319531 @default.
- W2353326121 hasConcept C150194340 @default.
- W2353326121 hasConcept C153911025 @default.
- W2353326121 hasConcept C168374021 @default.
- W2353326121 hasConcept C187882448 @default.
- W2353326121 hasConcept C19924922 @default.
- W2353326121 hasConcept C199360897 @default.
- W2353326121 hasConcept C202416437 @default.
- W2353326121 hasConcept C2779207651 @default.
- W2353326121 hasConcept C33161422 @default.
- W2353326121 hasConcept C40767141 @default.
- W2353326121 hasConcept C41008148 @default.
- W2353326121 hasConcept C54355233 @default.
- W2353326121 hasConcept C59822182 @default.
- W2353326121 hasConcept C79029880 @default.
- W2353326121 hasConcept C81089528 @default.
- W2353326121 hasConcept C86803240 @default.
- W2353326121 hasConceptScore W2353326121C104317684 @default.
- W2353326121 hasConceptScore W2353326121C121050878 @default.
- W2353326121 hasConceptScore W2353326121C128319531 @default.
- W2353326121 hasConceptScore W2353326121C150194340 @default.
- W2353326121 hasConceptScore W2353326121C153911025 @default.
- W2353326121 hasConceptScore W2353326121C168374021 @default.
- W2353326121 hasConceptScore W2353326121C187882448 @default.
- W2353326121 hasConceptScore W2353326121C19924922 @default.
- W2353326121 hasConceptScore W2353326121C199360897 @default.
- W2353326121 hasConceptScore W2353326121C202416437 @default.
- W2353326121 hasConceptScore W2353326121C2779207651 @default.
- W2353326121 hasConceptScore W2353326121C33161422 @default.
- W2353326121 hasConceptScore W2353326121C40767141 @default.
- W2353326121 hasConceptScore W2353326121C41008148 @default.
- W2353326121 hasConceptScore W2353326121C54355233 @default.
- W2353326121 hasConceptScore W2353326121C59822182 @default.
- W2353326121 hasConceptScore W2353326121C79029880 @default.
- W2353326121 hasConceptScore W2353326121C81089528 @default.
- W2353326121 hasConceptScore W2353326121C86803240 @default.
- W2353326121 hasLocation W23533261211 @default.
- W2353326121 hasOpenAccess W2353326121 @default.
- W2353326121 hasPrimaryLocation W23533261211 @default.
- W2353326121 hasRelatedWork W2347515328 @default.
- W2353326121 hasRelatedWork W2351944142 @default.
- W2353326121 hasRelatedWork W2364221357 @default.
- W2353326121 hasRelatedWork W2365169081 @default.
- W2353326121 hasRelatedWork W2366367904 @default.
- W2353326121 hasRelatedWork W2366473978 @default.
- W2353326121 hasRelatedWork W2368881403 @default.
- W2353326121 hasRelatedWork W2372046868 @default.
- W2353326121 hasRelatedWork W2372174243 @default.
- W2353326121 hasRelatedWork W2376249154 @default.
- W2353326121 hasRelatedWork W2379486258 @default.
- W2353326121 hasRelatedWork W2381195874 @default.
- W2353326121 hasRelatedWork W2384227396 @default.
- W2353326121 hasRelatedWork W2385223256 @default.
- W2353326121 hasRelatedWork W2385704299 @default.
- W2353326121 hasRelatedWork W2389626044 @default.
- W2353326121 hasRelatedWork W2389656439 @default.
- W2353326121 hasRelatedWork W2394351457 @default.
- W2353326121 hasRelatedWork W2412009672 @default.
- W2353326121 hasRelatedWork W2872359579 @default.
- W2353326121 isParatext "false" @default.
- W2353326121 isRetracted "false" @default.
- W2353326121 magId "2353326121" @default.
- W2353326121 workType "article" @default.