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- W2353343345 abstract "Aim:To investigate the effects of thermotherapy,chemotherapy(with paclitaxel) and thermochemotherapy on cell proliferation and MMP-2 mRNA expression of H446 cells.Methods:Different temperatures(39 ℃,40 ℃,41 ℃,42 ℃,43 ℃ and 44 ℃),different time(30 min,60 min and 90 min) of hyperthermia,and different doses(30 μg/L,60 μg/L,120 μg/L,240 μg/L and 480 μg/L) of paclitaxel were grouped respectively.MMT assay was adopted to measure cell proliferation and the inhibition rates.Semi-quantitive RT-PCR was applied to determine the expression of MMP-2 mRNA.Results and Conclusions:Paclitaxel and thermotherapy both could inhibit the growth of H446 cells and thermotherapy enhanced the inhibitive effects of paclitaxel on the H446 cell proliferation.The best group of temperature,time and paclitaxel concentration is thermotherapy at 43 ℃ for 90 min and 120 μg/L paclitaxel.Thermochemotherapy could evidently decrease the expression of MMP-2 mRNA." @default.
- W2353343345 created "2016-06-24" @default.
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- W2353343345 date "2008-01-01" @default.
- W2353343345 modified "2023-09-24" @default.
- W2353343345 title "Effects of thermotherapy on H446 cell growth and expression of MMP-2 mRNA in combination with paclitaxel" @default.
- W2353343345 hasPublicationYear "2008" @default.
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