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- W2353383723 abstract "Objective:To study chondrogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs) in monolayer culture in vitro Method:Human bone marrow was collected from three volunteer donors respectively The MSCs were isolated from the Percoll fraction of mononuclear cells and cell surface molecules were analyzed by flow cytometry BMSCs were cultured in inductive serum free medium containing transforming growth factor β 1 for 7 days,then examined by immunohistochemistry,in situ hybridization and alkaline phosphatase staining,and the proliferation of the cells was examined by 3H TdR incorporation Result:The adherent BMSCs expressed positive CD9,but negative CD34,CD38,CD45,CD61 After 7 days induction,the cells were slightly positively stained by alkaline phosphatase,immunohistochemistry and in situ hybridization analysis proved strong type Ⅱ collagen expression,but the incorporation of 3H TdR was lower than that of control( P 0 05 ) Conclusion:Human bone marrow derived mesenchymal stem cells can be induced to differentiate into chondrocytes in vitro,but the proliferation of the cells is low in serum free medium" @default.
- W2353383723 created "2016-06-24" @default.
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- W2353383723 date "2004-01-01" @default.
- W2353383723 modified "2023-09-23" @default.
- W2353383723 title "Chondrogenic differentiation of human bone marrow mesenchymal stem cells in vitro" @default.
- W2353383723 hasPublicationYear "2004" @default.
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