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- W2353438179 abstract "The expression vector pLEB590 and sulfide-quinone reductase gene( SQR) were digested by two restriction endonucleases. SQR gene was ligated into vector pLEB590-SQR by T4 DNA ligase,and the ligation products were transferred into Lactococcus lactis strain MG1363 by electroporation to get the recombinant vector. The recombinant vector pLEB590-SQR was verified by double enzyme digestion and PCR amplification. Then the verified pLEB590-SQR was transferred into MG1363 by electroporation and the positive clone was selected on the medium containing nisin. The expression of SQR was detected by SDS-PAGE and Western blotting. The results showed that the recombinant vector pLEB590-SQR was successfully constructed and the expression of SQR in MG1363 was detected. MG1363 carrying pLEB590-SQR presented a good genetic stability. L. lactis MG1363 could be used to express sulfide-quinone reductase( SQR)." @default.
- W2353438179 created "2016-06-24" @default.
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- W2353438179 date "2014-01-01" @default.
- W2353438179 modified "2023-09-27" @default.
- W2353438179 title "Expression of SQR gene in nisin-resistant Lactococcus lactis" @default.
- W2353438179 hasPublicationYear "2014" @default.
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