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- W2353455620 abstract "Objective To construct p3×flag-myc-CMV-24-HPV18E6 fusion gene in eukaryotic expression vector and test its expression in Hela cells.Methods HPV18E6 gene sequence with HindⅢ and SalⅠ restriction enzyme cutting site were amplified from total RNA of Hela cell line by reverse transcription-polymerase chain reaction (RT-PCR),then the HPV18E6 gene sequence was inserted into pMD-18T vector.After enzyme cutting by HindⅢ and SalⅠ,use T4 ligase to form the eukaryotic expression vector p3×flag-myc-CMV-24-HPV18E6.The plasmid p3×flag-myc-CMV-24-HPV18E6 were transfected into the Hela cells.Western-blot were used to test the expression levels of flag and HPV18E6 after 48 h.Results The p3×flag-myc-CMV-24-HPV18E6 eukaryotic expression vector was successfully constructed.Design of consistent seen after transfection of the expression of flag.Conclusion The eukaryotic expressing vector encoding HPV18 E6 was constructed and it expressed E6 protein correctly in Hela cells." @default.
- W2353455620 created "2016-06-24" @default.
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- W2353455620 date "2010-01-01" @default.
- W2353455620 modified "2023-09-24" @default.
- W2353455620 title "Construction of p3×flag-myc-CMV-24-HPV18E6 fusion gene expression vector and its expression in Hela Cells" @default.
- W2353455620 hasPublicationYear "2010" @default.
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