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- W2353469139 abstract "A VP2 gene of approximately 1 461 bp in length, which was obtained from the total RNA of chicken IBDV by RT-PCR, was cloned into the vector pET-28a to construct an expression vector pVP2. An expected protein of 53. 7 ku in size was expressed in E. coli BL21(DE3) induced by IPTG. SDS-PAGE and Western-blotting analyses showed that VP2 protein existed mainly in inclusion bodies and could react to anti-IBDV polyclonal and monoclonal antibodies. Then the protein was purified and refolded by lyso-zyme, Triton-100 and carbamide. Analysis using Dot-ELISA showed that activity of the renatured protein was increased by 10 times. The renatured protein was analysed with 16 monoclonal antibodies by Dot-ELISA. Result showed that the renatured protein could react strongly with 1H4,1E12,5B3 ,EA6 and 3C7, moderately with 4E4 and 1H11, and weakly with 4E5,3C4,1E11 and 3H9, but did not react with EC6, 3D12 and 1A1." @default.
- W2353469139 created "2016-06-24" @default.
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- W2353469139 date "2006-01-01" @default.
- W2353469139 modified "2023-09-23" @default.
- W2353469139 title "Prokaryotic expression of VP2 gene of infectious bursal disease virus H1 strain from chickens and renaturation of the expressed protein" @default.
- W2353469139 hasPublicationYear "2006" @default.
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