Matches in SemOpenAlex for { <https://semopenalex.org/work/W2353470138> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W2353470138 endingPage "6" @default.
- W2353470138 startingPage "11" @default.
- W2353470138 abstract "To obtain and characterize the single-stranded DNA (ssDNA) aptamers of human recombinant S100A8 protein.According to systematic evolution of ligands by exponential enrichment (SELEX) method, an ssDNA random library was subjected to 11 rounds of selection against human recombinant S100A8 protein. Using a biotin-strepavidin-HRP detecting system, the binding capacity of ssDNA to targeted protein from each round was monitored, until the binding level reached a saturation state. Then the ssDNA from the last cycle were cloned and sequenced, and the sequences were further analyzed by programs of bioinformatics.After 11 cycles of selection, 30 clones were selected randomly and sent to sequence analysis. A unique conserved sequence was not obtained among the 30 aptamers by the primary structure analysis, but three aptamers were found identical with three other aptamers respectively. The secondary structure analysis revealed that stem-loop and pocket were the main motifs, indicating that they may play a key role in the binding of aptamers to human recombinant S100A8 protein. According to the ratio of stem-loop to pocket, 30 aptamers were divided into 4 groups, and the affinity of aptamer No. 35 from group I was the highest.Aptamers against human recombinant S100A8 protein were identified by SELEX method, which laid the basis for the further application of the aptamers and the function study of human recombinant S100A8 protein." @default.
- W2353470138 created "2016-06-24" @default.
- W2353470138 creator A5006651636 @default.
- W2353470138 creator A5055110046 @default.
- W2353470138 creator A5091703689 @default.
- W2353470138 date "2012-02-18" @default.
- W2353470138 modified "2023-09-27" @default.
- W2353470138 title "[Screening and structure analysis of the aptamers against human recombinant S100A8 protein]." @default.
- W2353470138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22353892" @default.
- W2353470138 hasPublicationYear "2012" @default.
- W2353470138 type Work @default.
- W2353470138 sameAs 2353470138 @default.
- W2353470138 citedByCount "0" @default.
- W2353470138 crossrefType "journal-article" @default.
- W2353470138 hasAuthorship W2353470138A5006651636 @default.
- W2353470138 hasAuthorship W2353470138A5055110046 @default.
- W2353470138 hasAuthorship W2353470138A5091703689 @default.
- W2353470138 hasConcept C104317684 @default.
- W2353470138 hasConcept C122682173 @default.
- W2353470138 hasConcept C128972844 @default.
- W2353470138 hasConcept C153911025 @default.
- W2353470138 hasConcept C2780430158 @default.
- W2353470138 hasConcept C40767141 @default.
- W2353470138 hasConcept C54355233 @default.
- W2353470138 hasConcept C67705224 @default.
- W2353470138 hasConcept C70721500 @default.
- W2353470138 hasConcept C86803240 @default.
- W2353470138 hasConceptScore W2353470138C104317684 @default.
- W2353470138 hasConceptScore W2353470138C122682173 @default.
- W2353470138 hasConceptScore W2353470138C128972844 @default.
- W2353470138 hasConceptScore W2353470138C153911025 @default.
- W2353470138 hasConceptScore W2353470138C2780430158 @default.
- W2353470138 hasConceptScore W2353470138C40767141 @default.
- W2353470138 hasConceptScore W2353470138C54355233 @default.
- W2353470138 hasConceptScore W2353470138C67705224 @default.
- W2353470138 hasConceptScore W2353470138C70721500 @default.
- W2353470138 hasConceptScore W2353470138C86803240 @default.
- W2353470138 hasIssue "1" @default.
- W2353470138 hasLocation W23534701381 @default.
- W2353470138 hasOpenAccess W2353470138 @default.
- W2353470138 hasPrimaryLocation W23534701381 @default.
- W2353470138 hasRelatedWork W1487881598 @default.
- W2353470138 hasRelatedWork W1990993077 @default.
- W2353470138 hasRelatedWork W2114783261 @default.
- W2353470138 hasRelatedWork W2151839185 @default.
- W2353470138 hasRelatedWork W2345593164 @default.
- W2353470138 hasRelatedWork W2353470138 @default.
- W2353470138 hasRelatedWork W2800255143 @default.
- W2353470138 hasRelatedWork W2952517342 @default.
- W2353470138 hasRelatedWork W2989433860 @default.
- W2353470138 hasRelatedWork W59526245 @default.
- W2353470138 hasVolume "44" @default.
- W2353470138 isParatext "false" @default.
- W2353470138 isRetracted "false" @default.
- W2353470138 magId "2353470138" @default.
- W2353470138 workType "article" @default.