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- W2353485258 abstract "We have demonstrated the cell free MDCC MSB1 culture supernatant could inhibit the Marek's disease virus(MDV)propagation on CEF in vitro while promote oncogenicity of Jing 1 MDV challenged Chicken in vitro.In order to investigate which component playing the functional roles,1000 rpm centrifugated cell free MDCC MSB1 Culture supernatants were digested with RNase,DNase and proteinase K respectively and used to inoculate CEF culture together with MDV'814'.Thase above cell free supernatants were also centrifugated by 10000 rpm.The collected sediments were digested with Rnase,Dnase and proteinase K respectively too,and then used to inoculate SPF chickens together with Jing 1 MDV challenge.The results revealed that only the Dnase digested cell free supernatants and sediments respectively lost the inhibition effects of MDV propagation in vitro and the promotion effects of oncogenicity in vitro.The existence of DNA in the cell free supernatant was verified by gel electrophoresis analysis." @default.
- W2353485258 created "2016-06-24" @default.
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- W2353485258 date "1999-01-01" @default.
- W2353485258 modified "2023-09-25" @default.
- W2353485258 title "Determination on the Action of DNA in Cell free MDCC MSB1 Culture Supernatant to Marek's Disease in vitro and vivo" @default.
- W2353485258 hasPublicationYear "1999" @default.
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