Matches in SemOpenAlex for { <https://semopenalex.org/work/W2353597029> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2353597029 abstract "Objective To enhance the specificity and sensitivity of PCR assay of Cryptosporidium oocysts,so as to provide the basis for detection of Cryptosporidiosis epidemic. Methods A number of Cryptosporidium parvum oocysts from infected BALB/c mice were obtained by amplification method.The fecal materials of mice were collected from which oocysts were isolated and purified with discontinuous sucrose gradients technique.The oocysts were cracked by freezing and thawing method,as well as sonic cracking method.Extracted DNA was amplified by PCR targeting a 450 bp fragment of the conservative gene 18S rRNA.The PCR products were checked by the Agarose Gel Electrophoresis.Results The cracking rate of oocysts by freezing and thawing method(86%) was lower than that by sonic cracking method(92%).The extracted DNA template by both methods could amplify the target fragment,but the detection sensitivity by sonic method was higher than that by freezing and thawing method (detection limit:2 oocysts/mL vs 20 oocysts/mL).Conclusion The cracking effect of sonic cracking method on oocysts is better than that of freezing and thawing.The modified PCR assay of Cryptosporidium parvum was characterized by its better sensitivity and specificity." @default.
- W2353597029 created "2016-06-24" @default.
- W2353597029 creator A5028993654 @default.
- W2353597029 date "2009-01-01" @default.
- W2353597029 modified "2023-09-23" @default.
- W2353597029 title "GENOMIC DNA EXTRACTION FROM CRYPTOSPORIDIUM PARVUM OOCYSTS AND PCR DETECTION BASED ON TARGET GENE 18S rRNA" @default.
- W2353597029 hasPublicationYear "2009" @default.
- W2353597029 type Work @default.
- W2353597029 sameAs 2353597029 @default.
- W2353597029 citedByCount "0" @default.
- W2353597029 crossrefType "journal-article" @default.
- W2353597029 hasAuthorship W2353597029A5028993654 @default.
- W2353597029 hasConcept C104317684 @default.
- W2353597029 hasConcept C153911025 @default.
- W2353597029 hasConcept C17757408 @default.
- W2353597029 hasConcept C185592680 @default.
- W2353597029 hasConcept C2777795018 @default.
- W2353597029 hasConcept C2778271344 @default.
- W2353597029 hasConcept C2778877831 @default.
- W2353597029 hasConcept C43617362 @default.
- W2353597029 hasConcept C47698986 @default.
- W2353597029 hasConcept C49105822 @default.
- W2353597029 hasConcept C54355233 @default.
- W2353597029 hasConcept C552990157 @default.
- W2353597029 hasConcept C61716771 @default.
- W2353597029 hasConcept C67905577 @default.
- W2353597029 hasConcept C78063203 @default.
- W2353597029 hasConcept C86803240 @default.
- W2353597029 hasConcept C89423630 @default.
- W2353597029 hasConceptScore W2353597029C104317684 @default.
- W2353597029 hasConceptScore W2353597029C153911025 @default.
- W2353597029 hasConceptScore W2353597029C17757408 @default.
- W2353597029 hasConceptScore W2353597029C185592680 @default.
- W2353597029 hasConceptScore W2353597029C2777795018 @default.
- W2353597029 hasConceptScore W2353597029C2778271344 @default.
- W2353597029 hasConceptScore W2353597029C2778877831 @default.
- W2353597029 hasConceptScore W2353597029C43617362 @default.
- W2353597029 hasConceptScore W2353597029C47698986 @default.
- W2353597029 hasConceptScore W2353597029C49105822 @default.
- W2353597029 hasConceptScore W2353597029C54355233 @default.
- W2353597029 hasConceptScore W2353597029C552990157 @default.
- W2353597029 hasConceptScore W2353597029C61716771 @default.
- W2353597029 hasConceptScore W2353597029C67905577 @default.
- W2353597029 hasConceptScore W2353597029C78063203 @default.
- W2353597029 hasConceptScore W2353597029C86803240 @default.
- W2353597029 hasConceptScore W2353597029C89423630 @default.
- W2353597029 hasLocation W23535970291 @default.
- W2353597029 hasOpenAccess W2353597029 @default.
- W2353597029 hasPrimaryLocation W23535970291 @default.
- W2353597029 hasRelatedWork W153432171 @default.
- W2353597029 hasRelatedWork W1890372978 @default.
- W2353597029 hasRelatedWork W2031341474 @default.
- W2353597029 hasRelatedWork W2042996296 @default.
- W2353597029 hasRelatedWork W2052442257 @default.
- W2353597029 hasRelatedWork W2071899476 @default.
- W2353597029 hasRelatedWork W2080804058 @default.
- W2353597029 hasRelatedWork W2097398431 @default.
- W2353597029 hasRelatedWork W2110716033 @default.
- W2353597029 hasRelatedWork W2113475228 @default.
- W2353597029 hasRelatedWork W2123096652 @default.
- W2353597029 hasRelatedWork W2162942810 @default.
- W2353597029 hasRelatedWork W2347849942 @default.
- W2353597029 hasRelatedWork W2368916007 @default.
- W2353597029 hasRelatedWork W2379086036 @default.
- W2353597029 hasRelatedWork W2899272285 @default.
- W2353597029 hasRelatedWork W2903144672 @default.
- W2353597029 hasRelatedWork W3030908710 @default.
- W2353597029 hasRelatedWork W3143625435 @default.
- W2353597029 hasRelatedWork W2031951339 @default.
- W2353597029 isParatext "false" @default.
- W2353597029 isRetracted "false" @default.
- W2353597029 magId "2353597029" @default.
- W2353597029 workType "article" @default.