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- W2353604267 abstract "OBJECTIVE To establish a HPLC method for the simultaneous determination of amoxicillin and clavulanate in human serum. METHODS Serum samples were pretreated with benzaldehyde, then separated on the Discovery C18 column (5 μm, 150 mm×4.6 mm) at 291 with MeOH- H2O = 55:45 as the mobile phase at a flow rate of 1.0 mL·min-1. The fluorescence detection wavelengths were: λex = 386 nm and λem = 460 nm. RESULTS Clavulanate and amoxicillin were eluted at about 5.10 min and 6.20 min, respectively. The calibration curves were linear(r = 0.9999) from 0.062 to 4.968 mg·L-1 for clavulanate and from 0.089 to 35.718 mg·L-1 for amoxicillin, respectively. CONCLUSION The method is sensitive, accurate, reproducible, and suitable for the pharmacokinetic studies of amoxicillin sodium and clavulanate potassium formulations." @default.
- W2353604267 created "2016-06-24" @default.
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- W2353604267 date "2004-01-01" @default.
- W2353604267 modified "2023-09-26" @default.
- W2353604267 title "Simultaneous determination of serum concentration of amoxicillin and clavulanate by HPLC with fluorescence detection" @default.
- W2353604267 hasPublicationYear "2004" @default.
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